Recombinant Caldesmon HMW 抗体 (CF®488A)
Quick Overview for Recombinant Caldesmon HMW 抗体 (CF®488A) (ABIN6162481)
抗原
抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Recombinant Rabbit Monoclonal anti-Caldesmon, HMW (CALD1/1424R), CF488A Conjugate
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产品特性
- This antibody recognizes a protein of 150 kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant human full-length CALD1 protein
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亚型
- IgG kappa
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anti-Caldesmon HMW antibody
适用: 人, 大鼠 FACS, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Caldesmon HMW antibody适用: 人, 大鼠 FACS, IF, IHC (p) 宿主: 兔 Monoclonal CALD1-1424R unconjugated Recombinant Antibody
anti-Caldesmon HMW antibody适用: 人, 大鼠 FACS, IF, IHC (p) 宿主: 兔 Monoclonal CALD1-1424R unconjugated Recombinant Antibody
anti-Caldesmon HMW antibody适用: 人, 大鼠 FACS, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistology (formalin) 1-2 μg/mL
- for 10-20 min followed by cooling at RT for 20 min
- Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA
- pH 7.5-8.5
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Optimal dilution for a specific application should be determined by user
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说明
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Uterus, Blood vessels in all tissues, smooth muscle or leiomyosarcoma
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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- Caldesmon HMW
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别名
- Caldesmon, HMW
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背景
- CAD, CALD1, Caldesmon 1 Isoform 1, Caldesmon 1 Isoform 2, Caldesmon 1 Isoform 3, Caldesmon 1 Isoform 4, Caldesmon 1 Isoform 5, CDM, HCAD, LCAD, NAG22
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分子量
- 150 kDa
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基因ID
- 800, 490203
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UniProt
- Q05682
抗原
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